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Detection of Phytophthora, Pythium, Globisporangium, Hyaloperonospora and Plasmopara species in High-Throughput Sequencing data by in silico and in vitro analysis using Microbe Finder (MiFi®)
Species identification of plant pathogenic oomycetes based on morphology is challenging because of the limited number of variable …
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Development and evaluation of Microbe Finder (MiFi)®: a novel in silico diagnostic platform for pathogen detection from metagenomic data
Introduction. With expanding demand for diagnostics, newer methodologies are needed for faster, user-friendly and multiplexed pathogen …
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Development and Validation of E-Probes with the MiFi System for Detection of Ralstonia solanacearum Species Complex in Blueberries
The blueberry industry, a fast-growing industry in Florida, is threatened by bacterial wilt, caused by the Ralstonia solanacearum …
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Knowledge Gaps, Research Needs, and Opportunities in Plant Disease Diagnostic Assay Development and Validation
Sequence-based identification and detection technologies have revolutionized plant disease diagnostics in the last few decades, opening …
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Development and statistical validation of e-probe diagnostic nucleic acid analysis (EDNA) detection assays for the detection of citrus pathogens from raw high throughput sequencing data
The cost for high-throughput sequencing (HTS) has decreased significantly and has made it possible for the application of this …
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A Step Towards Validation of High-Throughput Sequencing for the Identification of Plant Pathogenic Oomycetes
The advancement in high-throughput sequencing (HTS) technology allows the detection of pathogens without the need for isolation or …
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TASPERT: Target-Specific Reverse Transcript Pools to Improve HTS Plant Virus Diagnostics
High-throughput sequencing (HTS) is becoming the new norm of diagnostics in plant quarantine settings. HTS can be used to detect, in …
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Genome analysis of Spiroplasma citri strains from different host plants and its leafhopper vectors
BACKGROUND: Spiroplasma citri comprises a bacterial complex that cause diseases in citrus, horseradish, carrot, sesame, and also …
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Microbe Finder (MiFi$^®$): Implementation of an Interactive Pathogen Detection Tool in Metagenomic Sequence Data
Agricultural high throughput diagnostics need to be fast, accurate and have multiplexing capacity. Metagenomic sequencing is being …
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Determining Limit of Detection of High Throughput Sequencing Diagnostics with MiFi®
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